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Originally published as JHC exPRESS on November 13, 2006.
doi:10.1369/jhc.6A7093.2006
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Journal of Histochemistry and Cytochemistry
Volume 55 (3): 235-245, 2007
Copyright ©The Histochemical Society, Inc.

A Subset of p23 Localized on Secretory Granules in Pancreatic ß-cells

Masahiro Hosaka1, Tsuyoshi Watanabe1, Yoshio Yamauchi, Yuko Sakai, Masayuki Suda, Shin Mizutani, Toshiyuki Takeuchi, Toshiaki Isobe and Tetsuro Izumi

Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan (MH,MS,SM,TT,TIzumi); Department of Anatomy II, Asahikawa Medical College, Asahikawa, Japan (TW,YS); and Integrated Proteomics System Project, Pioneer Research on Genome the Frontier, MEXT (YY,TIsobe) and Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University (TIsobe), Hachioji, Japan

Correspondence to: Dr. Tetsuro Izumi, Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi 371-8512, Japan. E-mail: tizumi{at}showa.gunma-u.ac.jp

Proteins on the membrane of secretory granules (SGs) involved in their biogenesis and exocytosis are poorly characterized compared with those of synaptic vesicle in neurons. Thus the secretory granule membrane was prepared from a mouse pancreatic ß-cell line MIN6 by subcellular fractionation, and protein constituents were analyzed by microscale two-dimensional liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Using this proteomics approach, one of the p24 family proteins, p23, was unexpectedly found in the granule fraction, although p24 proteins are generally regarded as functioning in the early secretory pathways between the endoplasmic reticulum and the Golgi apparatus. We further showed that p23 is expressed at high levels in endocrine cells. Furthermore, immunocytochemical analyses of pancreatic ß-cells at the light and electron microscopic levels demonstrated that a significant amount of p23 is localized on the insulin granule membrane, although it is most intensely concentrated at the cis-Golgi compartment as previously shown in non-endocrine cells. These findings suggest that a fraction of p23 enters post-Golgi compartments and may function in the biogenesis and/or quality control of SGs. (J Histochem Cytochem 55:235–245, 2007)

Key Words: p23 • secretory granules • mass spectrometry • pancreatic ß-cells


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