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Localization of Sulfonylurea Receptor Subunits, SUR2A and SUR2B, in Rat Heart

Ming Zhou, Hui-Jing He, Ryoji Suzuki, Ke-Xiang Liu, Osamu Tanaka, Masaki Sekiguchi, Hideaki Itoh, Katsumasa Kawahara and Hiroshi Abe

Department of Anatomy (MZ,H-JH,RS,HA), Department of Cardiovascular Surgery (K-XL), School of Medicine, and Department of Material-process Engineering and Applied Chemistry for Environment, Faculty of Engineering and Resource Science (HI), Akita University, Akita, Japan; Department of Morphology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa, Japan (OT,MS); and Department of Physiology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan (KK)

Correspondence to: Ming Zhou, MD, PhD, Department of Anatomy, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan. E-mail: mzhou{at}med.akita-u.ac.jp

To understand the possible functions and subcellular localizations of sulfonylurea receptors (SURs) in cardiac muscle, polyclonal anti-SUR2A and anti-SUR2B antisera were raised. Immunoblots revealed both SUR2A and SUR2B expression in mitochondrial fractions of rat heart and other cellular fractions such as microsomes and cell membranes. Immunostaining detected ubiquitous expression of both SUR2A and SUR2B in rat heart in the atria, ventricles, interatrial and interventricular septa, and smooth muscles and endothelia of the coronary arteries. Electron microscopy revealed SUR2A immunoreactivity in the cell membrane, endoplasmic reticulum (ER), and mitochondria. SUR2B immunoreactivity was mainly localized in the mitochondria as well as in the ER and cell membrane. Thus, SUR2A and SUR2B are not only the regulatory subunits of sarcolemmal K_ATP channels but may also function as regulatory subunits in mitochondrial K_ATP channels and play important roles in cardioprotection. (J Histochem Cytochem 55:795–804, 2007)

Key Words: SUR2A • SUR2B • immunoelectron microscopy • heart • rat

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