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Aberrant Expression of Histo-blood Group A Type 3 Antigens in Vascular Endothelial Cells in Inflammatory Sites

Mizuho Nosaka, Yuko Ishida, Aki Tanaka, Takahito Hayashi, Tomoko Miyashita, Chikako Kaminaka, Wolfgang Eisenmenger, Fukumi Furukawa and Akihiko Kimura

Department of Forensic Medicine (MN,YI,TH,TM,AK) and Department of Dermatology (AT,CK,FF), Wakayama Medical University, Wakayama, Japan, and Institute of Legal Medicine, University of Munich, Munich, Germany (WE)

Correspondence to: Akihiko Kimura, PhD, Department of Forensic Medicine, Wakayama Medical University, Kimiidera 811-1, 641-8509 Wakayama, Japan. E-mail: legkim{at}wakayama-med.ac.jp

Histo-blood group ABH antigens are widely distributed in human tissues. The epitopes of ABH antigens are carried by at least four different peripheral core isotypes of internal carbohydrate backbones (type 1–4). Each type of ABH antigen is expressed tissue specifically, and aberrant expression of ABH antigens is often observed during oncogenesis. We immunohistochemically examined the expression of A type 3 antigens in wounded and diseased skin tissues (A and AB blood groups). In uninjured skin, the expression of A type 3 antigens was restricted to the eccrine sweat gland. In addition to the sweat glands, A type 3 antigens were found in vascular endothelial cells of the wound sites. The extent of A type 3 antigens expression related to postinfliction intervals. A significantly higher expression rate of A type 3 antigens in endothelial cells was also observed in diseased skin, suggesting that inflammation might induce A type 3 antigen expression in endothelial cells. Double-color immunofluorescence staining of the specimens showed that von Willebrand factor (vWF) was a core-protein of A type 3 determinants aberrantly expressed in endothelial cells in inflamed tissues, suggesting that aberrant expression of A type 3 antigens is involved in stabilization of vWF in inflammation. (J Histochem Cytochem 56:223–231, 2008)

Key Words: histo-blood group A type 3 antigen • vascular endothelial cell • wound healing • inflammation • von Willebrand factor

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