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Originally published as JHC exPRESS on November 26, 2007.
doi:10.1369/jhc.7A7308.2007
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Journal of Histochemistry and Cytochemistry
Volume 56 (3): 233-241, 2008
Copyright ©The Histochemical Society, Inc.

Dendritic Cell Populations in Colon and Mesenteric Lymph Nodes of Patients With Crohn's Disease

Marleen I. Verstege, Fiebo J.W. ten Kate, Susanne M. Reinartz, Cornelis M. van Drunen, Frederik J.M. Slors, Willem A. Bemelman, Florry A. Vyth-Dreese and Anje A. te Velde

Centre for Experimental and Molecular Medicine (MIV,AAtV), Department of Pathology (FJWtK), Department of Otorhinolaryngology (SMR,CMvD), and Department of Surgery, Academic Medical Centre, Amsterdam, The Netherlands (FJMS,WAB), and Department of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands (FAV-D)

Correspondence to: Marleen I. Verstege, Academic Medical Centre/University of Amsterdam, Centre for Experimental and Molecular Medicine G2-132, PO Box 22700, 1100 DE Amsterdam, The Netherlands. E-mail: m.i.verstege{at}amc.uva.nl

Dendritic cells (DCs) are key cells in innate and adaptive immune responses that determine the pathophysiology of Crohn's disease. Intestinal DCs migrate from the mucosa into mesenteric lymph nodes (MLNs). A number of different markers are described to define the DC populations. In this study we have identified the phenotype and localization of intestinal and MLN DCs in patients with Crohn's disease and non-IBD patients based on these markers. We used immunohistochemistry to demonstrate that all markers (S-100, CD83, DC-SIGN, BDCA1-4, and CD1a) showed a different staining pattern varying from localization in T-cell areas of lymph follicles around blood vessels or single cells in the lamina propria and in the MLN in the medullary cords and in the subcapsular sinuses around blood vessels and in the T-cell areas. In conclusion, all different DC markers give variable staining patterns so there is no marker for the DC. (J Histochem Cytochem 56:233–241, 2008)

Key Words: Crohn's disease • dendritic cell markers • immunohistochemistry


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