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Immunolocalization of a Novel Collectin CL-K1 in Murine Tissues

Wataru Motomura, Takayuki Yoshizaki, Katsuki Ohtani, Toshikatsu Okumura, Mituko Fukuda, Jun Fukuzawa, Kenichiro Mori, Seong-Jae Jang, Naoki Nomura, Itsuro Yoshida, Yasuhiko Suzuki, Yutaka Kohgo and Nobutaka Wakamiya

Department of Microbiology and Immunochemistry (WM,TY,KO,MF,JF,KM,S-JJ,NN,IY,NW), Department of General Medicine (TO), and Division of Gastroenterology and Hematology Oncology (YK), Asahikawa Medical College, Asahikawa, Japan, and Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan (YS)

Correspondence to: Wataru Motomura, MD, Department of Microbiology and Immunochemistry, Asahikawa Medical College, 2-1-1-1Midorigaoka-Higashi, Asahikawa 078-8510, Japan. E-mail: momu{at}asahikawa-med.ac.jp

We have recently identified a novel collectin, CL-K1, that may play a role in innate immunity as a member of the collectin family. In this study using mice, we investigated the tissue distribution of CL-K1 for better understanding of its pathophysiological relevance. Real-time PCR analyses demonstrated that CL-K1 mRNA was expressed in all tissues tested. Immunohistochemical analyses demonstrated that CL-K1 was expressed in proximal tubules of kidney, in mucosa of the gastrointestinal tract, and in bronchial glands of bronchioles similar to the localization of SP-A and SP-D in these pulmonary structures. Immunohistochemistry also showed that CL-K1 was highly expressed in hepatocytes around the central veins in liver, which suggests that murine CL-K1 may be mainly produced in the liver and secreted into the blood stream as is human CL-K1. CL-K1 was especially detected in vascular smooth muscle in several types of tissues. In addition, it was also expressed in intestinal Paneth cells, in mesangial cells of kidney, in pancreatic islet D cells, and in neurons of the brain. It is of interest that this profile of CL-K1 expression is unique among the collectins. Together these histological findings may be useful for understanding the biological function of this novel collectin. (J Histochem Cytochem 56:243–252, 2008)

Key Words: CL-K1 • Colec11 • collectin • mannin-binding lectin • mouse • somatostatin • Paneth cells

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