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Originally published as JHC exPRESS on December 10, 2007.
doi:10.1369/jhc.7A7340.2007
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Journal of Histochemistry and Cytochemistry
Volume 56 (6): 531-538, 2008
Copyright ©The Histochemical Society, Inc.

Preparation of Polyclonal Antibody Specific for BRD7 and Detection of Its Expression Pattern in the Human Fetus

Huaying Liu1, Xiaoling Li1, Zhaoxia Niu1, Liming Zhang1, Ming Zhou, He Huang, Jiajin He, Wenling Zhang, Lan Xiao, Yunlian Tang, Li Wang and Guiyuan Li

Cancer Research Institute (HL,XL,ZN,LZ,MZ,JH,WZ,LX,YT,LW,GL) and Department of Histology and Embryology (HH), Xiang-Ya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China

Correspondence to: Guiyuan Li, Cancer Research Institute, Central South University, 110 Xiang-Ya Road, Changsha, Hunan 410078, People's Republic of China. E-mail: ligy{at}xysm.net

BRD7 is a novel bromodomain gene. It plays critical role in cell growth, cell cycle progression, and signal-dependent gene expression. Overexpression of the BRD7 gene in nasopharyngeal carcinoma cells is effective to inhibit cell growth and cell cycle progression from G1 to S phase. However, little is known about its bio-functions because of the unavailability of a specific BRD7 antibody. In this study, for the first time, we generated a highly specific BRD7 antibody. It is able to specifically recognize recombinant GST-BRD7N protein with a molecular mass of 65 kDa and recognize BRD7-Myc and endogenously expressed BRD7 protein with an approximate molecular mass of 75 kDa, which corresponds well with the calculated molecular mass of the BRD7 protein. More importantly, with these antisera, we analyzed BRD7 distribution in the human fetus by Western blot and immunohistochemistry assays. Obvious nuclear expression of BRD7 protein presents in human cerebellum, pancreas, intestines, liver, and kidney. Cardiomyocyte shows high cytoplasm expression of the BRD7 protein. Weak nuclear expression of the BRD7 protein is found in human cerebrum, lung, and stomach. These data may help to further study the cellular role of the BRD7 gene. In particular, the prepared BRD7 antibody will be helpful for studying the bio-functions of endogenously expressed BRD7 protein. (J Histochem Cytochem 56:531–538, 2008)

Key Words: bromodomain • BRD7 • antibody • human fetus • tissue distribution


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