Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
Originally published as JHC exPRESS on March 31, 2008.
doi:10.1369/jhc.2008.950873
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data for this article
Right arrow All Versions of this Article:
jhc.2008.950873v1
56/6/615    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Oberley, T. D.
Right arrow Articles by Kregel, K. C.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oberley, T. D.
Right arrow Articles by Kregel, K. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry
Volume 56 (6): 615-627, 2008
Copyright ©The Histochemical Society, Inc.

Aging Results in Increased Autophagy of Mitochondria and Protein Nitration in Rat Hepatocytes Following Heat Stress

Terry D. Oberley, Jamie M. Swanlund, Hannah J. Zhang and Kevin C. Kregel

Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin (TDO); Department of Pathology and Laboratory Medicine, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin (TDO,JMS); and Department of Integrative Physiology and Free Radical and Radiation Biology Program, Department of Radiation Oncology, The University of Iowa, Iowa City, Iowa (HJZ,KCK)

Correspondence to: Dr. Terry D. Oberley, MD, PhD, Department of Pathology and Laboratory Medicine, William S. Middleton Memorial Veterans Hospital, Room A-35, 2500 Overlook Terrace, Madison, WI 53705. E-mail: toberley{at}wisc.edu. Co-corresponding author: Dr. Kevin C. Kregel. E-mail: kevin-kregel{at}uiowa.edu

The natural breakdown of cells, tissues, and organ systems is a significant consequence of aging and is at least partially caused by a decreased ability to tolerate environmental stressors. Based on quantitative ultrastructural analysis using transmission electron microscopy and computer imaging, we show significant differences in hepatocyte morphology between young and old rats during a 48-hr recovery period following a 2-day heat stress protocol. Mitochondrial injury was greater overall in old compared with young rats. Autophagy was observed in both young and old rats, with autophagy greater overall in old compared with young hepatocytes. Lipid peroxidation and protein nitration were evaluated by localization and quantification of 4-hydroxy-2-nonenal (4-HNE)–modified protein adducts and 3-nitrotyrosine (3-NT) levels, respectively. Levels of 3-NT but not 4-HNE-protein adducts were significantly elevated in hepatocytes of old rats in comparison with young at 90 min after heat stress, suggesting a major role for reactive nitrogen species in the pathology observed at this time point. These results show a differential response of hepatocyte mitochondria to heat stress with aging, as well as greater levels of both autophagic and nitrative damage in old vs young hepatocytes. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials. (J Histochem Cytochem 56:615–627, 2008)

Key Words: mitochondria • 3-nitrotyrosine • autophagy • heat stress • oxidative stress


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2008