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Originally published as JHC exPRESS on April 28, 2008.
doi:10.1369/jhc.2008.950519
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Journal of Histochemistry and Cytochemistry
Volume 56 (8): 765-772, 2008
Copyright ©The Histochemical Society, Inc.

Expression of Calcyclin-binding Protein/Siah-1 Interacting Protein in Normal and Malignant Human Tissues: An Immunohistochemical Survey

Huihong Zhai1, Yongquan Shi1, Haifeng Jin, Yuanfei Li, Yuanyuan Lu, Xiong Chen, Jinbo Wang, Liping Ding, Xin Wang and Daiming Fan

State Key Laboratory of Cancer Biology, Institute of Digestive Diseases (HZ,YS,HJ,YLu,XC,JW,XW,DF) and Department of Pathology (YLi), Xijing Hospital, Fourth Military Medical University, Xi'an, China, and Department of Cardiovascular Medicine, the Second Artillery General Hospital, Beijing, China (LD)

Correspondence to: Daiming M. Fan, State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. E-mail: fandaim{at}fmmu.edu.cn

Calcyclin-binding protein (CacyBP)/Siah-1 interacting protein (SIP), a component of ubiquitin-mediated proteolysis, could bind the Skp1-Cul1-F box protein complex. Although CacyBP/SIP was implicated in p53-induced β-catenin degradation, its exact function was still unknown. Our previous studies showed that CacyBP/SIP could modulate the multidrug-resistant phenotype of gastric cancer cells and was highly expressed in gastric cancer tissues compared with that in non-cancerous tissues. In this study, CacyBP/SIP protein expression profile in a broad range of human normal tissues and carcinomas was analyzed by immunohistochemistry staining with anti-CacyBP/SIP monoclonal antibody first produced in our laboratory. CacyBP/SIP was generally localized in the cytoplasm/nucleus. Positive staining of CacyBP/SIP was found in brain, heart, lymph node, and esophagus. Weak staining was shown in the rectum and kidney. No CacyBP/SIP was detected in other normal tissues. However, CacyBP/SIP was ubiquitously detected in all kinds of tumor tissues and was highly expressed in nasopharyngeal carcinoma, osteogenic sarcoma, and pancreatic cancer. To our knowledge, this is the first study on the CacyBP/SIP expression pattern in a broad range of human normal and tumor tissues. The data presented should serve as a useful reference for other investigators in future studies of CacyBP/SIP functions. Hopefully, this knowledge will lead to discovery of more roles of CacyBP/SIP in tumorigenesis. (J Histochem Cytochem 56:765–772, 2008)

Key Words: calcyclin-binding protein/Siah-1 interacting protein • immunohistochemistry • tissue distribution • tumor tissue • normal tissue


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