This Article Full Text Full Text (PDF) All Versions of this Article: jhc.2008.950543v1 56/8/773    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Satoh, H. Articles by Takeya, M. PubMed PubMed Citation Articles by Satoh, H. Articles by Takeya, M. Social Bookmarking                      What's this?

Expression and Localization of Lectin-like Oxidized Low-density Lipoprotein Receptor-1 (LOX-1) in Murine and Human Placentas

Hiroo Satoh, Emi Kiyota, Yasuhiro Terasaki, Tatsuya Sawamura, Katsumasa Takagi, Hiroshi Mizuta and Motohiro Takeya

Department of Cell Pathology (HS,EK,YT,MT) and Department of Orthopaedic and Neuro-Musculoskeletal Surgery (HS,KT,HM), Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan, and Department of Vascular Physiology, National Cardiovascular Center Research Institute, Osaka, Japan (TS)

Correspondence to: Motohiro Takeya, Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan. E-mail: takeya{at}kumamoto-u.ac.jp

Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is one of the scavenger receptors that recognizes oxidized low-density lipoprotein as a major ligand. The placenta is a major source of prooxidant during pregnancy, and the level of placental oxidative stress increases rapidly at the end of the first trimester and tapers off later in gestation. In our study, we evaluated placental expression of LOX-1 during different gestational stages in mice and humans. We used immunohistochemistry and ISH to identify LOX-1–expressing cells in murine and human placentas. In both species, higher expression of LOX-1 mRNA during early to midgestational stages compared with late gestation—corresponding to the increased oxidative stress in early pregnancy—was shown by real-time RT-PCR. In murine placenta, we showed that LOX-1–expressing cells were fibroblast-like stromal cells in metrial glands and decidua basalis and that they were glycogen trophoblast cells in the junctional and labyrinth zones. In the human, LOX-1 expression was detected in villous cytotrophoblasts in both first trimester and term placentas. These localization patterns of LOX-1 in murine and human placentas suggest the possible involvement of LOX-1 in high oxidative stress conditions of pregnancy. (J Histochem Cytochem 56:773–784, 2008)

Key Words: LOX-1 • placenta • glycogen trophoblast cells • fibroblast-like stromal cells

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2008