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A NEW CARBOWAX METHOD FOR ROUTINELY PERFORMING LIPID, HEMATOXYLIN AND EOSIN AND ELASTIC STAINING TECHNIQUES ON ADJACENT FREEZE-DRIED OR FORMALIN-FIXED SECTIONS

FREDERICK T. ZUGIBE 1, KRYSTYNA C. KOPACZYK 1, WILLIAM E. CAPE 1, and JULES H. LAST 1

1 Geriatrics Research Project, Veterans' Administration Hospital, Downey, Ill., and the Department of Medicine, Northwestern University Medical School, Chicago, Illinois

1. A freeze-drying method is presented for the simultaneous processing of a large number of tissue specimens.

2. An infiltration medium consisting of carbowax "1000" and a blocking mixture consisting of carbowax "1000" and carbowax "4000" were developed and resulted in ribbons which could be cut as fine as 2 µ.

3. Diffusion currents encountered during flotation were obviated by the use of a General Flotation Solution consisting of potassium dichromate, glycerin and gelatin for routine sections and a Special Flotation Solution consisting of carbowax and glycerin for certain fragile tissues.

4. The application of routine stains to adjacent carbowax sections included a hematoxylin and cosin technique with good cosin differentiation and sharp hematoxylin staining, an elastic-collagen technique demonstrating distinct elastic and collagen fibers and a lipid technique with minimal fat loss.

5. This carbowax method allows for correlative, multiple staining and ultramicrochemical analysis on adjacent tissue sections.

Submitted on October 9, 1957


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A Radiopaque-Plastic Injection Technique for Assessing the Coronary Arteries and the Vasculature of Other Organs
Angiology, January 1, 1968; 19(1): 25 - 35.
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