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IMPROVED TECHNIQUE FOR THE HISTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE IN BRAIN TISSUE

REINHARD FRIEDE 1

1 From the Clinic of Neurosurgery, University of Freiburg, Germany

A tissue cutting technique for the histochemical demonstration of succinic dehydrogenase activity in brain tissue is described. The demonstration of succinic dehydrogenase in fine and delicate structures depends on a tissue temperature of –3 to –2°C. during cutting. Artefacts such as coarse granules, loss of activity and diffusion of stain are prevented by minimizing of traumatic effects due to this improved technique.

Submitted on March 13, 1958


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