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HISTOCHEMICAL DEMONSTRATION OF ACID PHOSPHATASE ACTIVITY IN OSTEOCLASTS

M. S. BURSTONE 1

1 National Institute of Dental Research, National Institutes of Health, Bethesda 14, Maryland.

High acid phosphatase activity was observed in osteoclasts of several species using a reproducible azo-dye technique.

High activity of two distinct enzymes, acid and alkaline phosphatase, are associated with osteoclasts and osteoblasts respectivey.

Althouth frozen-dried tissues are recommended for definitive studies, the enzyme techniques used give satisfactory results with cold acetone-fixed tissues. The most accurate localization of acid phosphatase in osteoclasts in controlled comparative studies is obtained with double-embedded frozen-dried undecalcified tissues in conjunction with naphthol AS-phosphates.

Submitted on June 16, 1958


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