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DETERMINATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN TISSUE CULTURE

NATHAN KAUFMAN 1 and ROBERT HILL 1

1 From the Department of Pathology, Western Reserve University School of Medicine at Cleveland Metropolitan General Hospital (formerly City Hospital), Cleveland, Ohio

Two methods of demonstrating succinic dehydrogenase activity in tissue cultures of HeLa cells have been demonstrated. One method employs a protective layer of agar applied to the cells prior to incubation in the selected substrate. The other method uses a mixture of agar and substrate which is placed on the cells; incubation follows without the further addition of substrate.

Submitted on October 21, 1958


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