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QUANTITATION OF PERIODIC ACID-SCHIFF POSITIVE SUBSTANCES USING RADIOACTIVE SCHIFF REAGENT

TIBOR BARKA 1 and LEONARD ORNSTEIN 1

1 Department of Pathology, and cell Research Laboratory, The Mount Sinai Hospital, New York 29, N. Y.

A simple method is described which makes it possible to determine the relative amount of Schiff reagent bound to periodic acid oxidized tissue sections. It is based on the application of S35-labeled Schiff reagent. It was found that paraffin embedded tissue sections bind significant amounts of Schiff reagent without any previous treatment, but using appropriate control sections it is possible to determine the relative amounts of aldehyde groups created by periodic acid oxidation as opposed to the amounts in the controls.

Submitted on April 10, 1959


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