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THE CORRELATION OF ULTRAVIOLET AND FEULGEN ABSORPTION MEASURED IN ASCITES TUMOR CELLS

TIBOR BARKA 1 and GUSTAV DALLNER 1

1 Institute for Cell Research and Genetics, Karolinska Institutet, Stockholm 60, Sweden, and Department of Pathology, The Mount Sinai Hospital, New York 29, N. Y.

The correlation of ultraviolet and Feulgen absorption after ribonuclease, cold perchloric acid, or ribonuclease plus perchloric acid treatments was investigated in fixed ascites tumor cells using microspectrophotometric methods. It was concluded from the measurements that the amount of ribonucleic acid resistant to 24 hour ribonuclease digestion is proportional to the amount of desoxyribonucleic acid revealed by Feulgen staining.

Submitted on April 27, 1959


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