Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ADAMS, C. W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ADAMS, C. W. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

OSMIUM TETROXIDE AND THE MARCHI METHOD: REACTIONS WITH POLAR AND NON-POLAR LIPIDS, PROTEIN AND POLYSACCHARIDE

C. W. M. ADAMS 1

1 Department of Pathology, Guy's Hospital Medical School, London, S.E. 1

1. The reactions of tissues with OsO4 and the Swank-Davenport KClO3-OsO4 (Marchi) reagent have been studied by blockade of the reducing groups of unsaturated lipid, protein and polysaccharide. Only blockade of the —CH=CH— bond prevented osmiophilia after brief staining (18 hr.).

2. Tissue-protein is neither stained nor demonstrably impregnated after brief treatment with OsO4.

3. Chromatographic separation of degenerating myelin revealed that the Marchi-substance is eluted in the esterified cholesterol fraction.

4. Unsaturated lipid but not protein or polysaccharide are stained by brief treatment with OsO4. The Marchi reaction distinguishes hydrophilic from hydrophobic unsaturated lipid.

Submitted on October 16, 1959


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. D. Litzlbauer, C. Neuhaeuser, A. Moell, S. Greschus, A. Breithecker, F. E. Franke, W. Kummer, and W. S. Rau
Three-dimensional imaging and morphometric analysis of alveolar tissue from microfocal X-ray-computed tomography
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L535 - L545.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. S. Hanker, A. R. Seaman, L. P. Weiss, H. Ueno, R. A. Bergman, and A. M. Seligman
Osmiophilic Reagents: New Cytochemical Principle for Light and Electron Microscopy
Science, November 20, 1964; 146(3647): 1039 - 1043.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1960