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THE HISTOCHEMICAL DEMONSTRATION OF agr-d-GLUCOSIDASE IN MAMMALIAN TISSUES

ALEXANDER M. RUTENBURG 1, JULIUS A. GOLDBARG 1, SELMA H. RUTENBURG 1, and RUTH T. LANG 1

1 Yamins Laboratory for Surgical Research and Departments of Surgery, Beth Israel Hospital and Harvard Medical School, Boston, Mass.

A histochemical method for the demonstration of agr-d-glucosidase is presented. The procedure and the problems encountered in its development are discussed. Surveys of mammalian tissues showed that this enzyme is generally demonstrable by this method only in the mucosa of the duodenum and upper jejunum and in the kidney cortex. Starvation resulted in decreased enzymatic activity in the intestinal mucosa, whereas increased activity was observed after the ingestion of food.

Submitted on November 20, 1959


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