Supplemental Material - Kwon et al. - Vol. 55, pp 575-584

Online Supplemental data for Kwon et al.: AVI Movie files.

Files in this Data Supplement:

• Kwon et al., Movie SM1 - Fluorescence microscopy of human renal OAT1 (green) in control tissue showing basolateral distribution in proximal tubule cells. The kidney tissue was dual stained with Texas red conjugated phalloidin for F-actin (red). Proximal tubule cells are identified by the presence of brush border. hOAT1 is present in all proximal tubule cells. Serial images were taken at 0.2 mm intervals throughout the 6 mm depth of tissue section using confocal microscopy. Three-dimensional reconstruction of these images was generated.
• Kwon et al., Movie SM2 - Fluorescence microscopy of hOAT1 in a human cadaveric renal allograft. The staining for hOAT1 is remarkable for diffuse large coarse cytoplasmic aggregates with patchy basal or almost no basolateral membrane staining. The cytoplasmic aggregates are variable in size. Serial images were taken at 0.2 mm intervals throughout the 6 mm depth of tissue section using confocal microscopy. Three-dimensional reconstruction of these images was generated.
• Kwon et al., Movie SM3 - Fluorescence microscopy of hOAT1 (green) in a human cadaveric renal allograft. The kidney tissue was dual stained with Texas red conjugated phalloidin for F-actin (red) to identify proximal tubule cells with brush border. Most of the staining for hOAT1 (green) is observed as fine diffuse cytoplasmic aggregates. Also diffuse cytoplasmic staining is above the level of background. Occasional patchy basal staining for hOAT1 is also noted as well as intraluminal aggregates. Interestingly, occasional patchy staining is also detected in peritubular intersititial area. Serial images were taken at 0.2 mm intervals throughout the 6 mm depth of tissue section using confocal microscopy. Three-dimensional reconstruction of these images was generated.