Supplemental Material - Heffer-Lauc et al. - Vol. 55, pp 805-812

Online Supplemental data for Heffer-Lauc et al.: Figure Image files.

Files in this Data Supplement:

• Heffer-Lauc et al., Supplemental Figure 1 - Immunohistochemistry of Thy-1 at room temperature Wild-type mouse brain sections were pre-incubated with different detergents for 2 h at room temperature and immunostained with anti-Thy-1 antibody. Significant extraction of Thy-1 was observed with all detergents.
• Heffer-Lauc et al., Supplemental Figure 2 - Immunohistochemistry of Thy-1 at 4ºC Wild-type mouse brain sections were pre-incubated with different detergents for 2 h at 4ºC and immunostained with anti-Thy-1 antibody. CHAPS, deoxycholic acid and Triton X-100 caused significant extraction and redistribution of Thy-1 from grey matter into the white matter of cerebellum, while other detergents (particularly Trappsol ,Tween 20, Brij 96V and Brij98) did not have this effect.
• Heffer-Lauc et al., Supplemental Figure 3 - Immunohistochemistry of GM1 at room temperature Wild-type mouse brain sections were pre-incubated with different detergents for 2 h at room temperature and immunostained with anti-GM1 antibody. Some of the detergents (deoxycholic acid and SDS) extracted significant amount of GM1 from tissue sections resulting in markedly reduced GM1 staining.
• Heffer-Lauc et al., Supplemental Figure 4 - Immunohistochemistry of MAG Wild-type mouse brain sections were pre-incubated with different detergents for 2 h at 4ºC, or extracted with chloroform/methanol before immunostaining with anti-MAG antibody. The figure clearly shows that pre-treatment with detergents or extraction with organic solvents is necessary for efficient immunostaining of MAG.