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JHC exPRESS: First Published April 28, 2008. doi:10.1369/jhc.2008.950519
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on August 1, 2008.
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Articles

Expression of CacyBP/SIP Protein in Normal and Malignant Human Tissues: An Immunohistochemical Survey

Huihong Zhai 1, Yongquan Shi 1, Haifeng Jin 1, Yuanfei Li 1, Yuanyuan Lu 1, Xiong Chen 1, Jinbo Wang 1, Liping Ding 1, Xin Wang 1 and Daiming Fan 1*

1 State Key Laboratory of Cancer Biology, Institute of Digestive Diseases (HZ,YS,HJ,YL,XC,JW,XW,DF) and Department of Pathology (YL), Xijing Hospital, Fourth Military Medical University, Xi’an, China, and Department of Cardiovascular, the Second Artillery General Hospital, Beijing, China (LD)

* To whom correspondence should be addressed. E-mail: fandaim{at}fmmu.edu.cn.

Submitted on December 6, 2007
Accepted on 17 April 2008


  Abstract
CacyBP/SIP, a component of the ubiquitin-mediated proteolysis, could bind SKP1-CUL1-F box protein complex. Although CacyBP/SIP was implicated in p53-induced {beta}-catenin degradation, its exact function was still unknown. Our previous studies showed that CacyBP/SIP could modulate multidrug-resistant phenotype of gastric cancer cells and highly expressed in gastric cancer tissues comparing to that in non-cancerous tissues. In the present study, CacyBP/SIP protein expression profile in a broad range of human normal tissues and carcinomas was analyzed by immunohistochemistry staining with anti-CacyBP/SIP monoclonal antibody firstly produced in our laboratory. CacyBP/SIP was generally localized in the cytoplasm/nucleus. Positive staining of CacyBP/SIP was found in brain, heart, lymph node and esophagus. Weak stain was shown in rectum, kidney. No CacyBP/SIP was detected in other normal tissues. However, CacyBP/SIP was ubiquitously detected in all kinds of tumor tissues, especially highly expressed in nasopharyngeal carcinoma, osteogenic sarcoma and pancreatic cancer. To our knowledge, this was the first study on CacyBP/SIP expression pattern in a broad range of human normal and tumor tissues. The data presented should serve as a useful reference for other investigators in future studies of CacyBP/SIP functions. It was hoped that this knowledge will lead to more discoveries of roles of CacyBP/SIP in tumorigenesis.

Key Words: CacyBP/SIP, immunohistochemistry, tissue distribution, tumor tissue, normal tissue


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