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JHC exPRESS: First Published November 26, 2007. doi:10.1369/jhc.7A7308.2007
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on March 1, 2008.
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Articles

Dendritic Cell Populations in Colon and Mesenteric Lymph Nodes of Patients With Crohn’s Disease

Marleen I. Verstege 1*, Fiebo J.W. ten Kate 1, Susanne M. Reinartz 1, Cornelis M. van Drunen 1, Frederik J.M. Slors 1, Willem A. Bemelman 1, Florry A. Vyth-Dreese 1 and Anje A. te Velde 1

1 Centre for Experimental and Molecular Medicine (MIV,AAtV), Department of Pathology (FJWtK), Department of Otorhinolaryngology (SMR,CMvD), and Department of Surgery (FJMS,WAB), Academic Medical Centre, Amsterdam, The Netherlands, and Department of Immunology, NKI-AVL, Amsterdam, The Netherlands (FAV-D)

* To whom correspondence should be addressed. E-mail: m.i.verstege{at}amc.uva.nl .

Submitted on July 6, 2007
Accepted on 4 November 2007


  Abstract
Dendritic cells (DCs) are key cells in innate and adaptive immune responses that determine the pathophysiology of Crohn’s disease. Intestinal DCs migrate from the mucosa into mesenteric lymph nodes (MLNs). A number of different markers are described to define the dendritic cell populations. In this study we have identified the phenotype and localization of intestinal and MLN dendritic cells in patients with Crohn’s disease and non-IBD patients based on these markers. We used immunohistochemistry to demonstrate that all the markers, s-100, CD83, DC-SIGN, BDCA1-4 and CD1a, showed a different staining pattern varying from localization in T cell areas of lymph follicles, around blood vessels or single cells in the lamina propria and in the MLN in the medullary cords, in the subcapsular sinuses, around blood vessels and in the T cell areas. In conclusion, all different dendritic cell markers give variable staining patterns: so there is no marker for the dendritic cell.

Key Words: Crohn’s disease, dendritic cell markers, immunohistochemistry


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