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JHC exPRESS: First Published November 26, 2007. doi:10.1369/jhc.7A7310.2007
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on March 1, 2008.
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Articles

Immunohistochemical Localization of Collagen Type XI {alpha}1 and {alpha}2 Chains in Human Colon Tissue

Kara B. Bowen 1, Aaron P. Reimers 1, Sarah Lumen 1, Joseph D. Kronz 1, William E. Fyffe 1 and Julia Thom Oxford 1*

1 Department of Biology, Biomolecular Research Center, Boise State University, Boise, Idaho (JTO); Department of Biology, Northwest Nazarene University, Nampa, Idaho (KBB,APR,SL,WEF); and Department of Pathology, Mercy Medical Center, Nampa, Idaho (JDK)

* To whom correspondence should be addressed. E-mail: joxford{at}boisestate.edu.

Submitted on July 9, 2007
Accepted on 8 November 2007


  Abstract
In previous studies, collagen XI mRNA has been detected in colon cancer, but its location in human colon tissue has not been determined. The heterotrimeric collagen XI consists of three alpha chains. While it is known that collagen XI plays a regulatory role in collagen fibril formation, its function in the colon is unknown. The characterization of normal human colon tissue will allow a better understand collagen XI’s variance in abnormal tissues. Grossly normal and malignant human colon tissue was obtained from pathology archives. Immunohistochemical staining with a 58K Golgi marker and {alpha}1(XI) and {alpha}2(XI) antisera was used to specifically locate their presence in normal colon tissue. A comparative bright field microscopic analysis demonstrated the presence of collagen XI in human colon. The juxtanuclear, dot-like collagen XI staining in the Golgi apparatus of the goblet cells in normal tissue paralleled the staining of the 58K Golgi marker. Ultra light microscopy verified these results. Staining was also confirmed in malignant colon tissue. This study is the first to demonstrate that collagen XI is present in the Golgi apparatus of normal human colon goblet cells and localize collagen XI in both normal and malignant tissue. Though the function of collagen XI in the colon is unknown, our immunohistochemical characterization provides the foundation for future immunohistopathology studies of the colon.

Key Words: collagen type XI, colon, immunohistochemistry, Golgi apparatus, 58K Golgi marker


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