Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
JHC exPRESS: First Published December 10, 2007. doi:10.1369/jhc.7A7340.2007
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on June 1, 2008.
This Article
Right arrow exPRESS PDF
Right arrow All Versions of this Article:
jhc.7A7340.2007v1
56/6/531    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, H.
Right arrow Articles by Li, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, H.
Right arrow Articles by Li, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Articles

Preparation of Polyclonal Antibody Specific for BRD7 and Detection of Its Expression Pattern in the Human Fetus

Huaying Liu 1, Xiaoling Li 1, Zhaoxia Niu 1, Liming Zhang 1, Ming Zhou 1, He Huang 1, Jiajin He 1, Wenling Zhang 1, Lan Xiao 1, Yunlian Tang 1, Li Wang 1 and Guiyuan Li 1*

1 Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Changsha, Hunan, People’s Republic of China

* To whom correspondence should be addressed. E-mail: ligy{at}xysm.net.

Submitted on August 11, 2007
Accepted on 26 November 2007


  Abstract
BRD7 is a novel bromodomain gene. It plays a critical role in cell growth, cell cycle progression, and signal-dependent gene expression. Over-expression of BRD7 gene in nasopharyngeal carcinoma cells is effective to inhibit cell growth and cell cycle progression from G1 to S phase. However, little is known about its biofunctions, due to the unavailablility of a specific BRD7 antibody. In the present study, we for the first time generated a highly specific BRD7 antibody. It is able to specifically recognize recombinant GST-BRD7N protein with the molecular weight of 65 KD, and recognize BRD7-Myc and endogenously expressed BRD7 protein with the approximate molecular weight of 75 KD, which corresponds well with the calculated molecular weight of BRD7 protein. More importantly, with these antisera, we analyzed BRD7 distribution in the human fetus by western blot and immunohistochemistry assays. Obvious nuclear expression of BRD7 protein presents in human cerebellum, pancreas, intestines, liver and kidney. Cardiomyocyte shows high cytoplasm expression of BRD7 protein. Weak nuclear expression of BRD7 protein is found in human cerebrum, lung and stomach. These data may help to further study the cellular role of BRD7 gene. In particular, the prepared BRD7 antibody will be helpful for investigating the bio-functions of endogenously expressed BRD7 protein.

Key Words: bromodain, BRD7, antibody, human fetus, tissue distribution


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2007